Catalog #5225

TeloCol®-6 is 50 mL of 6 mg/ml, type I bovine telocollagen solution for 2D coatings or 3D hydrogels. This kit comes with a neutralization solution for easy handling and preparation. Being a higher concentration telocollagen, TeloCol®-6 will form firm hydrogels.

TeloCol®-6 bovine collagen solution is derived from an acid extraction process yielding a telopeptide-intact collagen. The pro-peptide regions at both ends of the collagen chain, N- and C-telopeptide regions, are maintained.

TeloCol®-6 collagen is approximately 95% Type I collagen with the remainder being comprised of Type III collagen. Each product includes a bottle containing 50 ml of collagen solution accompanied with a bottle of pre-formulated neutralizing solution for the formation of a collagen gel. This product is supplied at a concentration of approximately 6 mg/ml. TeloCol®-6 is sterile filtered and provided in user-friendly packaging for use and storage.

TeloCol®-6 is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.

Parameter, Testing, and Method

TeloCol®-6 Type I Collagen #5225

Sterilization Method

Filtration

Extraction Method

Acid - telocollagen

Form

Solution

Package Size

50 mL Kit

Storage Temperature of Collagen

2-10°C

Storage Temperature of Neutralization Solution

Room Temperature

Shelf Life

Minimum of 6 months from date of receipt

Collagen Concentration - Biuret

5.8-6.8 mg/mL

Collagen Purity - Silver Staining

99%

pH

1.9-3.0

Kinetic Gel Test (Minutes)

40

Gel Formation Tube Test (Minutes)

40

Fibrillogenesis (Absorbance Units)

0.35

Electrophoretic Pattern - Coomassie Blue

Characteristic

Sterility - USP modified

No growth

Endotoxin - LAL

10.0 EU/mL

Osmolality (mOsmo H2O/kg)

35

Cell Attachment Assay

Pass

Source

Bovine Hide

Hydrogel Young s Modulus E (Pa)

Characteristic

References for TeloCol®:

Drzewiecki, Kathryn E., et al. \"Circular Dichroism Spectroscopy of Collagen Fibrillogenesis: A New Use for an Old Technique.” Biophysical Journal, vol. 111, no. 11, 2016, pp. 2377–2386., doi:10.1016/j.bpj.2016.10.023

Wingender, Brian, et al. \"Biomimetic Organization of Collagen Matrices to Template Bone-like Microstructures.” Matrix Biology, vol. 52-54, 2016, pp. 384–396., doi:10.1016/j.matbio.2016.02.004.

Burla, F., Tauber, J., Dussi, S., Gucht, J. V. D. Koenderink, G. H. Stress management in composite biopolymer networks. Nature Physics15,549–553 (2019).